9D5D
Crystal Structure of Blood Coagulation Factor VIII C2 Domain Mutant L2251A/L2252A
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2023-10-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 49.962, 49.962, 116.565 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.930 - 1.830 |
R-factor | 0.19 |
Rwork | 0.186 |
R-free | 0.22290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.067 |
Data reduction software | APEX |
Data scaling software | Aimless |
Phasing software | PHENIX (v1.21.1) |
Refinement software | PHENIX (v1.21.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.930 | 1.900 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.151 | 0.689 |
Rmeas | 0.155 | 0.730 |
Rpim | 0.032 | 0.231 |
Number of reflections | 13614 | 1248 |
<I/σ(I)> | 15.3 | 2.8 |
Completeness [%] | 99.2 | 93.06 |
Redundancy | 20.8 | 9.3 |
CC(1/2) | 0.998 | 0.841 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 4 M potassium formate, 0.1 M Bis-Tris propane, pH 9, 2% w/v PEG2000 MME |