Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-04 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.629, 80.755, 66.742 |
| Unit cell angles | 90.00, 106.19, 90.00 |
Refinement procedure
| Resolution | 47.783 - 2.100 |
| Rwork | 0.212 |
| R-free | 0.28550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.592 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.783 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.091 | 1.405 |
| Number of reflections | 36183 | 2947 |
| <I/σ(I)> | 7.4 | 0.8 |
| Completeness [%] | 98.6 | 98.4 |
| Redundancy | 7.5 | 7.7 |
| CC(1/2) | 0.996 | 0.657 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG 3350, 200 mM Ammonium tartrate dibasic |
