9D41
Crystal structure of N-terminal domain of Borealin (20-88) in complex with synthetic antibody fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-26 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920105 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 72.432, 72.432, 243.129 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.060 - 1.840 |
| R-factor | 0.15748 |
| Rwork | 0.155 |
| R-free | 0.19732 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.625 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.060 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rpim | 0.059 | 1.700 |
| Number of reflections | 65038 | 3190 |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 11.9 | |
| CC(1/2) | 0.999 | 0.353 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 292 | 0.1 M CAPS, 40 % v/v MPD |
