9D3J

Structure of L9 Fab in complex with CSP_Res5-Y_mC2 Scaffold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	119.936, 119.936, 217.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.900 - 3.500
R-factor	0.2771
Rwork	0.272
R-free	0.32250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.533
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.000	3.560
High resolution limit [Å]	3.500	3.500
Number of reflections	20805	1019
<I/σ(I)>	15.9
Completeness [%]	99.9
Redundancy	9.8
CC(1/2)	0.997	0.601

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.08 M Sodium Cacodylate pH 6.5, 0.16 M Calcium acetate, 20% Glycerol, 14.4% (wt/vol) PEG8000