Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-31 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 97.544, 97.544, 69.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.040 - 1.800 |
| R-factor | 0.1821 |
| Rwork | 0.181 |
| R-free | 0.20030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.037 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.630 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.088 | 1.287 |
| Number of reflections | 35586 | 2072 |
| <I/σ(I)> | 16.5 | 2.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.1 | |
| CC(1/2) | 0.999 | 0.756 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | PEG 4000, ammonium acetate, citrate |
