9D1Y

Structure of G75R Ubiquitin bound to KLHDC3-EloB/C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97741
Spacegroup name	C 2 2 21
Unit cell lengths	114.560, 121.543, 152.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.850 - 2.600
R-factor	0.1701
Rwork	0.168
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.921
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.146	0.062	0.782
Rmeas	0.156	0.066	0.830
Rpim	0.053	0.023	0.276
Number of reflections	33218	3498	3275
<I/σ(I)>	3.4
Completeness [%]	100.0	100	100
Redundancy	8.4	8	8.7
CC(1/2)	1.000	0.997	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	10%PEG5KMME, 5% Tacsimate pH=7.0, 0.1M HEPES pH=7.0