9D10

Photoactive Yellow Protein, crystals from PEG, room temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	293
Detector technology	PIXEL
Collection date	2023-02-03
Detector	PSI JUNGFRAU 4M
Wavelength(s)	1.1
Spacegroup name	P 65
Unit cell lengths	41.360, 41.360, 118.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.820 - 1.800
R-factor	0.1548
Rwork	0.152
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.819
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	118.000	1.850
High resolution limit [Å]	1.800	1.800
Number of reflections	2377266	513
<I/σ(I)>	5
Completeness [%]	100.0
Redundancy	135
CC(1/2)	0.990	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	6.5	292	100 mM MES, 40% PEG 4000