9D06
Crystal structure of IgG1 FC M252R at pH 5.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2024-01-22 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.972 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.385, 111.454, 63.660 |
| Unit cell angles | 90.00, 95.74, 90.00 |
Refinement procedure
| Resolution | 32.500 - 2.330 |
| R-factor | 0.2307 |
| Rwork | 0.225 |
| R-free | 0.27870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.106 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.520 | 3.350 |
| High resolution limit [Å] | 2.330 | 3.180 |
| Rmerge | 0.150 | 1.800 |
| Rmeas | 0.420 | 0.980 |
| Rpim | 0.170 | 0.774 |
| Number of reflections | 19846 | 1168 |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7 | 6.6 |
| CC(1/2) | 0.958 | 0.447 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | 0.1M Sodium citrate pH 5.6, 20% Propanol and 20% PEG 4K |
