9CZS

Structure of the self-association domain of LDB2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95374
Spacegroup name	P 21 21 2
Unit cell lengths	61.196, 147.634, 42.994
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.110 - 3.170
R-factor	0.2178
Rwork	0.210
R-free	0.29230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.300
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.210	3.200
High resolution limit [Å]	3.170	3.170
Number of reflections	12703	2289
<I/σ(I)>	4.9
Completeness [%]	99.7
Redundancy	1.8
CC(1/2)	0.976	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Ammonium sulfate, HEPES, PEG 8000, MPD