9CYS
Toxin/immunity complex for a T6SS lipase effector from E. cloacae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 99.081, 111.739, 119.337 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.920 - 1.750 |
R-factor | 0.1858 |
Rwork | 0.183 |
R-free | 0.21180 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.120 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.049 | 0.806 |
Rmeas | 0.054 | 0.935 |
Rpim | 0.028 | 0.938 |
Number of reflections | 66430 | 3499 |
<I/σ(I)> | 16.9 | 2 |
Completeness [%] | 99.1 | 96.5 |
Redundancy | 7.5 | 7.1 |
CC(1/2) | 0.999 | 0.792 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.4 M sodium citrate, 22% PEG3350 |