9CYN

Flagellar filament sheath protein FlaA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-23
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.979507
Spacegroup name	P 21 21 21
Unit cell lengths	81.417, 105.776, 108.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.820 - 1.820
R-factor	0.1856
Rwork	0.184
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.312
Data reduction software	XDS (20230630)
Data scaling software	Aimless (0.7.13)
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.820	1.848
High resolution limit [Å]	1.816	1.816
Rmerge	0.106	5.769
Rmeas	0.110	5.987
Rpim	0.030	1.596
Number of reflections	85228	4238
<I/σ(I)>	13.1	0.5
Completeness [%]	100.0	100
Redundancy	13.8	14
CC(1/2)	0.998	0.375

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	ammonium sulfate, sodium cacodylate