9CY6

Crystal structure of IgG1 FC M252H at pH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	110
Detector technology	PIXEL
Collection date	2023-12-04
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.972
Spacegroup name	P 21 21 21
Unit cell lengths	49.610, 79.055, 139.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.770 - 2.060
R-factor	0.2363
Rwork	0.234
R-free	0.28210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.033
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.770	3.540
High resolution limit [Å]	2.050	3.360
Rmerge	0.200	1.800
Rmeas	0.403	1.000
Rpim	0.116	0.990
Number of reflections	34638	2145
<I/σ(I)>	6.8
Completeness [%]	99.9
Redundancy	12.1	12
CC(1/2)	0.993	0.414

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M Bis-tris propane pH 7.5, and 8% PEG8K