9CXW

Crystal structure of Human FN3K bound with ADP and DMF (II)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	52.854, 111.765, 132.661
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.470 - 1.800
R-factor	0.1891
Rwork	0.188
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.666
Data reduction software	RAPD
Data scaling software	RAPD
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487:0000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	132.660	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.058
Number of reflections	73858	7274
<I/σ(I)>	19.2
Completeness [%]	99.8
Redundancy	7.5
CC(1/2)	0.990	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	-crystallized in 0.5 M ammonium sulfate, 0.1 M tri-sodium citrate, and 1 M lithium sulfate -Soaked in 5 mM phospho-DMF-ADP mixture -cryo-protected in 0.9X reservoir solution + 20% glycerol