9CXN

Crystal structure of Human FN3K bound with AMPPNP and DMF

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	52.600, 111.519, 132.915
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.460 - 1.900
R-factor	0.1784
Rwork	0.176
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.577
Data reduction software	RAPD
Data scaling software	RAPD
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487:0000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	132.900	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.043
Number of reflections	62294	6159
<I/σ(I)>	18.5
Completeness [%]	99.6
Redundancy	3.8
CC(1/2)	0.990	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.5 M ammonium sulfate, 0.1 M tri-sodium citrate, and 1 M lithium sulfate