9CX8
Crystal structure of Human FN3K in apo-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.351, 111.528, 132.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.350 - 1.670 |
| R-factor | 0.1763 |
| Rwork | 0.175 |
| R-free | 0.20480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.455 |
| Data reduction software | RAPD |
| Data scaling software | RAPD |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1-4487-0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 132.600 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.047 | 0.730 |
| Number of reflections | 90249 | 8938 |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.990 | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.5 M ammonium sulfate, 0.1 M tri-sodium citrate, and 1 M lithium sulfate |
