9CWN

NRIP1_133 / RIP140 SxxLxxLL motif coregulator peptide with agonist GW1929 and PPARg LBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	I 2 2 2
Unit cell lengths	57.236, 118.815, 151.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.570 - 2.700
R-factor	0.2001
Rwork	0.198
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.939
Data reduction software	XDS (Jun 30, 2023 (BUILT 20230630))
Data scaling software	STARANISO (2.3.79 (20211010))
Phasing software	PHENIX (1.19_4092)
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.570	2.797
High resolution limit [Å]	2.700	2.700
Rmerge	0.059	0.422
Rmeas	0.084	0.597
Rpim	0.059	0.422
Number of reflections	13880	948
<I/σ(I)>	10.63	2.15
Completeness [%]	94.7
Redundancy	2
CC(1/2)	0.995	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	1.6 M AmSO4, 100mM Tris pH 8.5