9CVA
Crystal structure of human ribokinase in complex with K+, Mg2+, and AMPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.380, 70.750, 90.650 |
| Unit cell angles | 90.00, 92.36, 90.00 |
Refinement procedure
| Resolution | 55.756 - 1.600 |
| R-factor | 0.1544 |
| Rwork | 0.153 |
| R-free | 0.18470 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.681 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.760 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.039 | 0.660 |
| Rmeas | 0.044 | 0.737 |
| Rpim | 0.019 | 0.435 |
| Number of reflections | 74579 | 3527 |
| <I/σ(I)> | 18.1 | 2.2 |
| Completeness [%] | 98.7 | 94.1 |
| Redundancy | 5 | 2.2 |
| CC(1/2) | 0.999 | 0.714 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277.15 | 0.1 M TrisHCl (pH 8.5), 25% (W/v) PEG 2000 MME |
