9CUG

X-ray Structure of human Interferon Regulatory Factor 4 (IRF4) IAD Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9201
Spacegroup name	P 21 21 21
Unit cell lengths	84.228, 87.427, 97.526
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.870 - 2.490
R-factor	0.2102
Rwork	0.208
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.841
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.870	2.584
High resolution limit [Å]	2.490	2.490
Number of reflections	25625	16237
<I/σ(I)>	5.71
Completeness [%]	99.6	98.77
Redundancy	6.3	6.5
CC(1/2)	0.982	0.449

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	25% PEG3350, 200 mM lithium sulfate and 100 mM Hepes, pH 7.5