9CUA
Human STING G230A/R293Q variant bound to cGAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 |
| Unit cell lengths | 109.810, 109.810, 35.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.660 - 2.220 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.110 | 2.340 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.054 | 0.719 |
| Number of reflections | 21288 | 3077 |
| <I/σ(I)> | 15.2 | 2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.5 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 1.1 M Sodium/Potassium Tartrate, 0.1 M MES pH 6.0 |
