9CU9
Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/L36K at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Agilent SuperNova |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2020-10-21 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.081, 60.256, 38.731 |
| Unit cell angles | 90.00, 94.75, 90.00 |
Refinement procedure
| Resolution | 23.260 - 1.900 |
| R-factor | 0.19084 |
| Rwork | 0.189 |
| R-free | 0.22240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.703 |
| Data reduction software | CrysalisPro (39.46) |
| Data scaling software | CrysalisPro (39.46) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.730 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.017 | 0.146 |
| Number of reflections | 11317 | 1138 |
| <I/σ(I)> | 53.12 | 4.81 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.1 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20% MPD, 25 mM Potassium Phosphate, |
