9CSX

matrix metalloproteinase-10 proenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	80.841, 72.003, 54.495
Unit cell angles	90.00, 130.82, 90.00

Refinement procedure

Resolution	27.120 - 1.670
R-factor	0.19
Rwork	0.189
R-free	0.21260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.968
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.621	1.697
High resolution limit [Å]	1.669	1.669
Rmerge	0.159	0.986
Rmeas	0.197
Rpim	0.115	0.731
Number of reflections	25894	1322
<I/σ(I)>	2.8	0.8
Completeness [%]	94.1
Redundancy	2.6
CC(1/2)	0.985	0.339

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.2 M zinc acetate, 0.1 M imidazole, pH 7.5 and 17 % (w/v)PEG 3000, 4.0 % (v/v) acetonitrile