9CSR

16mer self-complementary duplex RNA with s(2)C:I pair sequence 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2024-04-18
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.000026
Spacegroup name	H 3 2
Unit cell lengths	40.942, 40.942, 123.477
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.747 - 1.330
Rwork	0.188
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	2.348
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.350
High resolution limit [Å]	1.330	1.330
Rmerge	0.068	0.471
Rmeas	0.072	0.546
Rpim	0.026	0.267
Number of reflections	9369	427
<I/σ(I)>	21.6	2.5
Completeness [%]	98.4	92
Redundancy	7.8	3.7
CC(1/2)	0.978	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.0 M Ammonium sulfate, 5% v/v 2-Propanol