9CSQ

16mer self-complementary duplex RNA with s(2)C:I pair sequence 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2024-06-21
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.000050
Spacegroup name	H 3 2
Unit cell lengths	43.405, 43.405, 123.136
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.952 - 1.540
Rwork	0.222
R-free	0.24480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.481
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.050	1.570
High resolution limit [Å]	1.540	1.540
Rmerge	0.052	0.725
Rmeas	0.058	0.805
Rpim	0.025	0.346
Number of reflections	6923	339
<I/σ(I)>	19	2.5
Completeness [%]	99.9	100
Redundancy	9.4	9.9
CC(1/2)	0.998	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M Calcium acetate hydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 18% w/v Polyethylene glycol 8,000