9CSP

16mer self-complementary duplex RNA with s(2)C:G pair sequence 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2024-06-27
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.000050
Spacegroup name	H 3 2
Unit cell lengths	41.217, 41.217, 123.468
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.291 - 1.600
Rwork	0.176
R-free	0.26410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	2.261
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.106	0.254
Rmeas	0.115	0.293
Rpim	0.044	0.141
Number of reflections	5469	228
<I/σ(I)>	14.8	2.7
Completeness [%]	97.5	82.9
Redundancy	7.7	3.3
CC(1/2)	0.937	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, 30% v/v Polyethylene glycol 400