9CSF
Structure of a dimeric ubiquitin variant (UbV3) that inhibits the protease (PRO) from Turnip Yellow Mosaic Virus (TYMV)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2022-05-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 75.698, 75.698, 121.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.940 - 1.840 |
| R-factor | 0.1969 |
| Rwork | 0.195 |
| R-free | 0.23010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.903 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.940 | 1.910 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.058 | 0.932 |
| Rmeas | 0.060 | 0.969 |
| Rpim | 0.016 | 0.263 |
| Number of reflections | 15581 | 1476 |
| <I/σ(I)> | 28.72 | 2.86 |
| Completeness [%] | 99.7 | 97.87 |
| Redundancy | 13.8 | 13.2 |
| CC(1/2) | 1.000 | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15% PEG 1000, 0.5M Ammonium Sulfate |
