9CS7

Structure of Azospirillum bacterial CARD crystal form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-04-02
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	37.959, 65.368, 38.831
Unit cell angles	90.00, 111.07, 90.00

Refinement procedure

Resolution	36.230 - 1.880
R-factor	0.2014
Rwork	0.198
R-free	0.23210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.487
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.240	1.920
High resolution limit [Å]	1.880	1.880
Rpim	0.060	0.775
Number of reflections	14422	868
<I/σ(I)>	7.7	1.4
Completeness [%]	99.2
Redundancy	6.2
CC(1/2)	0.996	0.577

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM HEPES-KOH (pH 7.5), 200 mM ammonium acetate, and 25% PEG-3350