9CQD

Antibody 2B11 bound to the central conserved domain of RSV G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	74.654, 184.339, 161.236
Unit cell angles	90.00, 96.88, 90.00

Refinement procedure

Resolution	80.040 - 3.100
R-factor	0.2927
Rwork	0.292
R-free	0.32420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.878
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.170	3.150
High resolution limit [Å]	3.100	3.100
Number of reflections	78391	3862
<I/σ(I)>	3.3
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)	0.984	0.348

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	0.22M Ammonium Citrate Dibasic and 15% PEG 3350