9CQA

Structure of antibody 1G1 bound to the central conserved region of RSV G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033167
Spacegroup name	P 21 21 21
Unit cell lengths	76.418, 80.753, 175.181
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.320 - 1.740
R-factor	0.2111
Rwork	0.211
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.969
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.320	1.770
High resolution limit [Å]	1.740	1.740
Number of reflections	110905	5008
<I/σ(I)>	14.6
Completeness [%]	99.2
Redundancy	13.2
CC(1/2)	0.999	0.457

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	0.1M BIS-TRIS (pH 6.5), 0.2M Ammonium Sulfate, and 24.5% PEG 3350