9CQ5
Mn-bound RuBisCO from spinach with CABP inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 218.029, 218.893, 111.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.510 - 2.500 |
| R-factor | 0.1987 |
| Rwork | 0.197 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.042 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 154.470 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.560 | 5.222 |
| Rmeas | 0.606 | 5.610 |
| Rpim | 0.229 | 2.045 |
| Number of reflections | 252873 | 12416 |
| <I/σ(I)> | 5.9 | 0.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.5 |
| CC(1/2) | 0.983 | 0.332 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 298 | PEG 3350, lithium acetate, potassium phosphate |
