9CPZ
C387S variant of D-ornithine/D-lysine decarboxylase complexed with PMP and 4-guanidinobutanal
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.590, 49.110, 139.810 |
| Unit cell angles | 90.00, 115.80, 90.00 |
Refinement procedure
| Resolution | 48.920 - 1.540 |
| R-factor | 0.1687 |
| Rwork | 0.168 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.832 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.920 | 1.710 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Number of reflections | 83046 | 4154 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 92.5 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.995 | 0.528 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M HEPES-K, pH 7.5, 0.2-0.4 M sodium acetate, 20% PEG 3350 |
