9CP6

Crystal structure of the DNA binding domain of FLI1 (residues 259-371)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-12
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.97940
Spacegroup name	P 65 2 2
Unit cell lengths	44.584, 44.584, 175.704
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.000 - 1.660
R-factor	0.2041
Rwork	0.202
R-free	0.23542
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.368
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.690
High resolution limit [Å]	1.660	1.660
Rmerge	0.073
Number of reflections	13210	632
<I/σ(I)>	48
Completeness [%]	100.0
Redundancy	11.6	10.9
CC(1/2)	0.998	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	0.1 M Hepes, pH 6.5, 1 M Na citrate