9CMB

Human PU.1 ETS Domain (165-270) bound to d(AATAAAAGGAAGTGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.920119
Spacegroup name	C 1 2 1
Unit cell lengths	90.954, 102.731, 55.753
Unit cell angles	90.00, 111.65, 90.00

Refinement procedure

Resolution	28.910 - 2.150
R-factor	0.2342
Rwork	0.233
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.553
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.910	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.053	0.478
Rmeas	0.075	0.676
Rpim	0.053	0.478
Number of reflections	25670	2547
<I/σ(I)>	7.6	1.7
Completeness [%]	99.1	98.7
Redundancy	1.8	1.8
CC(1/2)	0.995	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	100 mM sodium cacodylate, pH 6.5, 200 mM zinc acetate and 2% PEG 600