Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 97.836, 97.836, 69.292 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.070 - 1.850 |
| R-factor | 0.1896 |
| Rwork | 0.188 |
| R-free | 0.21690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.726 |
| Data reduction software | autoPROC (1.0.5) |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.650 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.120 | 1.314 |
| Number of reflections | 33027 | 2006 |
| <I/σ(I)> | 15.3 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.4 | 14 |
| CC(1/2) | 0.999 | 0.768 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | PEG 4000, ammonium acetate, citrate |
