9CLG

Importin alpha isoform 2 with synthetic zero net-charge nuclear localization signal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.012, 89.964, 99.158
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.630 - 2.420
R-factor	0.16263
Rwork	0.161
R-free	0.20237
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.471
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.450
High resolution limit [Å]	2.410	6.540	2.410
Rmerge	0.072	0.021	0.483
Rmeas	0.079	0.023	0.526
Rpim	0.031	0.009	0.205
Total number of observations	174156
Number of reflections	27207	1498	1313
<I/σ(I)>	6.9
Completeness [%]	99.9	99.8	99.8
Redundancy	6.4	6.1	6.3
CC(1/2)	0.997	1.000	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	296	0.8 M NaCit, 0.1 M HEPES pH 6.2, 60% glycerol, 10 mM DTT