9CKX
Crystal structure of Dsk2 Sti1 domain bound to a transmembrane domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-21 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.410, 63.920, 67.430 |
| Unit cell angles | 90.00, 98.83, 90.00 |
Refinement procedure
| Resolution | 46.130 - 1.980 |
| R-factor | 0.2207 |
| Rwork | 0.220 |
| R-free | 0.23210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.800 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.130 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.056 | 0.827 |
| Rpim | 0.024 | 0.367 |
| Number of reflections | 36912 | 4887 |
| <I/σ(I)> | 17.7 | 2.1 |
| Completeness [%] | 93.3 | 85.9 |
| Redundancy | 6.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2.5% MPD, 100 mM Tris pH 8.5 |
