9CKQ

Human G protein-coupled receptor kinase 5-D311N ligand-free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	277
Detector technology	PIXEL
Collection date	2022-11-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	P 41 21 2
Unit cell lengths	137.272, 137.272, 70.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.670 - 2.700
R-factor	0.2394
Rwork	0.235
R-free	0.29410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.596
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.060	2.550
High resolution limit [Å]	2.430	2.490
Rmerge	0.144
Number of reflections	26003	2546
<I/σ(I)>	9.7
Completeness [%]	100.0
Redundancy	13
CC(1/2)		0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	crystallized in 200mM potassium citrate tribasic, 20% polyethylene glycol 3350, followed by soaking in 25% polyethylene glycol 3350, 10% Glycerol