9CKH

[d2d3-2Ag] Tensegrity triangle with deazapurines on the helical and center strands with two deazaA:Ag+:T base pairs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-23
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.979346
Spacegroup name	P 63
Unit cell lengths	123.277, 123.277, 62.265
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.670 - 5.920
R-factor	0.1621
Rwork	0.161
R-free	0.17930
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.453
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.670	19.670	6.737
High resolution limit [Å]	5.920	11.101	5.922
Number of reflections	2128	192	192
<I/σ(I)>	8.9	27.6	1.1
Completeness [%]	86.7
Redundancy	10.6
CC(1/2)	0.999	0.999	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM MOPS, 1.25 M magnesium sulfate