9CK2

Human PU.1 minimal ETS Domain (165-258) bound to d(AATAAGCGGAAGTGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977410
Spacegroup name	P 1 21 1
Unit cell lengths	42.871, 60.569, 44.292
Unit cell angles	90.00, 116.59, 90.00

Refinement procedure

Resolution	33.150 - 1.710
R-factor	0.1533
Rwork	0.150
R-free	0.18390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.244
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.150	1.770
High resolution limit [Å]	1.710	1.710
Rmerge	0.025	0.158
Rmeas	0.036	0.223
Rpim	0.025	0.158
Number of reflections	21885	2191
<I/σ(I)>	13.2	3.5
Completeness [%]	99.6	99.9
Redundancy	1.9	1.9
CC(1/2)	0.999	0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	2% PEG 3350, 100 mM Sodium Acetate, pH 4.6