9CJM
Red fluorescent protein mRuby3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.998, 101.494, 273.681 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.510 - 3.000 |
| R-factor | 0.2796 |
| Rwork | 0.278 |
| R-free | 0.30599 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.741 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.230 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 19445 | 2792 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 93.6 | |
| Redundancy | 5 | |
| CC(1/2) | 0.902 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | 0.2 M Ammonium Tartrate Dibasic, 20% (w/v) PEG 3350 |
