9CJ6

Crystal Structure of SARS-CoV-2 N-NTD with part of N-arm complex with ssDNA.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	I 41
Unit cell lengths	90.891, 90.891, 36.341
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.740 - 1.550
R-factor	0.1804
Rwork	0.179
R-free	0.20440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	13.854
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.610
High resolution limit [Å]	1.550	3.340	1.550
Rmerge	0.067	0.040	0.653
Rmeas	0.070	0.042	0.680
Rpim	0.019	0.012	0.186
Total number of observations	278517
Number of reflections	21606	2222	2097
<I/σ(I)>	10.8
Completeness [%]	99.8	99.4	98.6
Redundancy	12.9	12.8	11.6
CC(1/2)	0.998	0.999	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	277	0.2M HEPES, pH 7.4 + 20% PEG4000