9CIL
Crystal structure of Staphylococcal nuclease variant Delta+PHS T41V/S59A at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2023-06-28 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.021, 60.753, 38.429 |
| Unit cell angles | 90.00, 92.92, 90.00 |
Refinement procedure
| Resolution | 21.700 - 2.000 |
| R-factor | 0.1872 |
| Rwork | 0.185 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.772 |
| Data reduction software | CrysalisPro (42.80a 64-bit (release 13-01-2023)) |
| Data scaling software | CrysalisPro (42.80a 64-bit (release 13-01-2023)) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.920 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.013 | 0.094 |
| Number of reflections | 9707 | 799 |
| <I/σ(I)> | 76.43 | 11.37 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.4 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 35% MPD, 25 mM Potassium Phosphate, |
