9CH5

Structure of the alpha-N-methyltransferase (SonM) and RiPP precursor (SonA-L63D) heteromeric complex (bound to SAM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033167
Spacegroup name	P 1 21 1
Unit cell lengths	52.480, 108.380, 59.150
Unit cell angles	90.00, 93.67, 90.00

Refinement procedure

Resolution	19.900 - 2.000
R-factor	0.2418
Rwork	0.240
R-free	0.27940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.041
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	2.100
High resolution limit [Å]	2.000	2.000
Rmeas	0.077	0.473
Number of reflections	75115	10193
<I/σ(I)>	9.19
Completeness [%]	85.6
Redundancy	2.29
CC(1/2)	0.998	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	240 mM sodium malonate pH 5.5 with 2% PEG 3350