9CH1
Structure of the alpha-N-methyltransferase (SonM) and RiPP precursor (SonA-I65W) heteromeric complex (bound to SAM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033167 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.200, 59.100, 59.300 |
| Unit cell angles | 96.00, 112.40, 93.40 |
Refinement procedure
| Resolution | 29.220 - 2.100 |
| R-factor | 0.2116 |
| Rwork | 0.210 |
| R-free | 0.24889 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.655 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.220 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.080 | 0.327 |
| Number of reflections | 33547 | 4543 |
| <I/σ(I)> | 10.64 | |
| Completeness [%] | 88.5 | |
| Redundancy | 2.29 | |
| CC(1/2) | 0.995 | 0.911 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 240 mM sodium malonate pH 5.5 with 14% PEG 3350 |
