9CH1

Structure of the alpha-N-methyltransferase (SonM) and RiPP precursor (SonA-I65W) heteromeric complex (bound to SAM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033167
Spacegroup name	P 1
Unit cell lengths	52.200, 59.100, 59.300
Unit cell angles	96.00, 112.40, 93.40

Refinement procedure

Resolution	29.220 - 2.100
R-factor	0.2116
Rwork	0.210
R-free	0.24889
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.655
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.220	2.200
High resolution limit [Å]	2.100	2.100
Rmeas	0.080	0.327
Number of reflections	33547	4543
<I/σ(I)>	10.64
Completeness [%]	88.5
Redundancy	2.29
CC(1/2)	0.995	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	240 mM sodium malonate pH 5.5 with 14% PEG 3350