9CGW

Structure of the alpha-N-methyltransferase (SonM) and RiPP precursor (SonA-I65W) heteromeric complex (no cofactor)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 32 2 1
Unit cell lengths	91.810, 91.810, 161.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.900 - 2.650
R-factor	0.22429
Rwork	0.221
R-free	0.28019
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.574
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.900	2.750
High resolution limit [Å]	2.650	2.650
Rmerge	0.155
Rmeas		1.717
Number of reflections	23018	2397
<I/σ(I)>	13.39
Completeness [%]	99.7
Redundancy	7.4
CC(1/2)	0.998	0.504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	240 mM sodium malonate pH 5.5 with 10% PEG 3350