Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.124, 105.858, 114.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.050 - 3.100 |
| R-factor | 0.1834 |
| Rwork | 0.181 |
| R-free | 0.23280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.044 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.050 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 1.340 | |
| Number of reflections | 13573 | 5979 |
| <I/σ(I)> | 5.8 | 1.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.980 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1.2 M LiCl, 25% (w/v) PEG 4000, 100 mM HEPES pH 8.5 |
