9CGH

Photoactive yellow protein crystallized in situ on cyclic olefin copolymer microfluidic chip through counter diffusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	293
Detector technology	PIXEL
Collection date	2023-12-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979460
Spacegroup name	P 65
Unit cell lengths	41.194, 41.194, 117.819
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.680 - 1.320
R-factor	0.1715
Rwork	0.171
R-free	0.17900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.949
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.680	1.340
High resolution limit [Å]	1.320	1.320
Number of reflections	26412	1232
<I/σ(I)>	23
Completeness [%]	99.6	93.26
Redundancy	13.8
CC(1/2)	0.999	0.437

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION		293	PEG 4000, 2-Morpholinoethanesulphonic acid