9CGH
Photoactive yellow protein crystallized in situ on cyclic olefin copolymer microfluidic chip through counter diffusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2023-12-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979460 |
Spacegroup name | P 65 |
Unit cell lengths | 41.194, 41.194, 117.819 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.680 - 1.320 |
R-factor | 0.1715 |
Rwork | 0.171 |
R-free | 0.17900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.949 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.680 | 1.340 |
High resolution limit [Å] | 1.320 | 1.320 |
Number of reflections | 26412 | 1232 |
<I/σ(I)> | 23 | |
Completeness [%] | 99.6 | 93.26 |
Redundancy | 13.8 | |
CC(1/2) | 0.999 | 0.437 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | COUNTER-DIFFUSION | 293 | PEG 4000, 2-Morpholinoethanesulphonic acid |