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9CGH

Photoactive yellow protein crystallized in situ on cyclic olefin copolymer microfluidic chip through counter diffusion

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL12-1
Synchrotron siteSSRL
BeamlineBL12-1
Temperature [K]293
Detector technologyPIXEL
Collection date2023-12-11
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.979460
Spacegroup nameP 65
Unit cell lengths41.194, 41.194, 117.819
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution35.680 - 1.320
R-factor0.1715
Rwork0.171
R-free0.17900
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.007
RMSD bond angle0.949
Data reduction softwarexia2
Data scaling softwarexia2
Phasing softwarePHENIX
Refinement softwarePHENIX (1.19.2)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]35.6801.340
High resolution limit [Å]1.3201.320
Number of reflections264121232
<I/σ(I)>23
Completeness [%]99.693.26
Redundancy13.8
CC(1/2)0.9990.437
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1COUNTER-DIFFUSION293PEG 4000, 2-Morpholinoethanesulphonic acid

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