9CG8

CRYSTAL STRUCTURE OF THE P285S VARIANT OF SERINE HYDROXYMETHYLTRANSFERASE 8 FROM SOYBEAN CULTIVAR FORREST

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2023-10-05
Detector	RDI CMOS_8M
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	174.175, 174.175, 183.658
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.500 - 1.900
R-factor	0.2291
Rwork	0.227
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.989
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.127	2.561
Rpim	0.045	0.946
Number of reflections	251510	12415
<I/σ(I)>	13.4	1.2
Completeness [%]	100.0	100
Redundancy	10	9.5
CC(1/2)	0.997	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2 % v/v tacsimate 0.1 M Hepes, pH 7.5 20% w/v PEG 3350