9CFA
Germline-targeting HIV-1 gp120 engineered outer domain eODgt8 in complex with Fab eOD-CL04.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.818, 159.333, 101.057 |
| Unit cell angles | 90.00, 108.23, 90.00 |
Refinement procedure
| Resolution | 48.220 - 3.060 |
| R-factor | 0.2257 |
| Rwork | 0.224 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.593 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.220 | 3.150 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Rmerge | 0.238 | 2.000 |
| Rmeas | 0.271 | 2.350 |
| Rpim | 0.126 | 1.200 |
| Number of reflections | 42163 | 2073 |
| <I/σ(I)> | 8.3 | 0.9 |
| Completeness [%] | 96.0 | 95.8 |
| Redundancy | 4.4 | 3.4 |
| CC(1/2) | 0.981 | 0.315 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M sodium dihydrogen phosphate, 20% Peg3350 |
