9CF6
Germline-targeting HIV-1 gp120 engineered outer domain eODgt8 in complex with Fab eOD-CL02.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.039, 72.601, 93.817 |
| Unit cell angles | 90.00, 107.52, 90.00 |
Refinement procedure
| Resolution | 39.883 - 2.700 |
| Rwork | 0.276 |
| R-free | 0.30610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.131 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.883 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.158 | 1.040 |
| Rmeas | 0.174 | 1.150 |
| Rpim | 0.072 | 0.473 |
| Number of reflections | 18286 | 2448 |
| <I/σ(I)> | 7.7 | 1.6 |
| Completeness [%] | 98.3 | 99.4 |
| Redundancy | 5.6 | 5.9 |
| CC(1/2) | 0.988 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M magnesium nitrate, 20% Peg3350, 15% ethylene glycol |
