9CEI

Human DJ-1, no mixing, pink beam time-resolved serial crystallography

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	293
Detector technology	CCD
Collection date	2022-11-10
Detector	RAYONIX MX340-HS
Wavelength(s)	1.240-1.016
Spacegroup name	P 31 2 1
Unit cell lengths	76.030, 76.030, 76.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.840 - 1.770
R-factor	0.2058
Rwork	0.204
R-free	0.23500
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	0.734
Data reduction software	Precognition
Data scaling software	careless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.840	1.830
High resolution limit [Å]	1.770	1.770
Number of reflections	25220	2459
<I/σ(I)>	17.21	2.22
Completeness [%]	99.8	99.84
Redundancy	65.1	21.9
CC(1/2)	0.980	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.5	293	100 mM HEPES pH=7.5, 200 mM NaCl, and 15% PEG 3350